Isolation of antigenic variants of leptospiras from puppies and pigs experimentally infected with Leptospira interrogans Serovars canicola and pomona.
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چکیده
منابع مشابه
Isolation of leptospira Serovars Canicola and Copenhageni from cattle urine in the state of ParanÁ, Brazil
In 2001, 698 urine samples were randomly collected from cattle at a slaughterhouse in the State of Paraná, Brazil. Direct examination using dark field microscopy was carried out immediately after collection. Five putative positive samples were cultured in modified EMJH medium, yielding two positive cultures (LO-14 and LO-10). Typing with monoclonal antibodies revealed that the two isolates were...
متن کاملExperimental canine leptospirosis caused by Leptospira interrogans serovars pomona and bratislava.
OBJECTIVE To evaluate gross, histopathologic, and serum biochemical findings caused by Leptospira interrogans serovars pomona and bratislava inoculated in dogs. ANIMALS Twenty-seven 8-week-old female Beagles. PROCEDURE Dogs were randomly assigned to challenge or control groups. Challenge groups were conjunctivally inoculated on 3 successive days with 5 x 10(7) L interrogans serovar pomona (...
متن کاملIsolation and Characterization of Two Novel Plasmids from Pathogenic Leptospira interrogans Serogroup Canicola Serovar Canicola Strain Gui44
BACKGROUND Previous genomic analysis of pathogenic Leptospira has identified two circular chromosomes but no plasmid. This study aims to investigate potential extrachromosomal elements of L.interrogans serovar Canicola strain Gui44. METHODOLOGY Two novel plasmids, pGui1 and pGui2, were isolated from the pathogenic strain Gui44, using a modified alkaline lysis method. Southern blotting was per...
متن کاملCopurification of Leptospira interrogans serovar pomona hemolysin and sphingomyelinase C.
The hemolytic and sphingomyelinase C activities of supernatants of cultures of Leptospira interrogans serovar pomona tended to copurify when isoelectric fractionation was carried out. Both activities focused primarily at pH 8.1. Considered in conjunction with other circumstantial evidence, the results led to the conclusion that sphingomyelinase C is responsible for hemolysis.
متن کاملLeptospira interrogans serovar pomona associated with abortion in cattle: isolation methods and laboratory animal histopathology.
Leptospira interrogans serovar pomona was successfully isolated from cattle urine in the western Transvaal after an abortion storm had occurred. Direct inoculation of EMJH medium proved the most successful method. The selective agent, 5-fluorouracil, was most effective in controlling contamination when used at the 0,4 mg/ml level. The strain isolated was pathogenic in hamsters, but specific les...
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ژورنال
عنوان ژورنال: The Japanese Journal of Veterinary Science
سال: 1980
ISSN: 0021-5295,1881-1442
DOI: 10.1292/jvms1939.42.177